Sari Defi Okzelia Pengarang

ABSTRAK Sonneratia caseolaris (L.) Engl. or commonly called pidada fruit is one type of mangrove that is commonly found in the coastal areas of Muara Gembong, Bekasi, West Java. Pidada contains vitamins and secondary metabolite compounds that are beneficial for health. The purpose of this study was to determine the concentration of ethanolic extract, n-hexane fraction, ethyl acetate fraction, and butanol fraction of pidada fruit that could inhibit 50% absorbance of DPPH as free radical. Extraction was conducted by maceration method using 96% ethanol, then fractionated using n-hexane, ethyl acetate and butanol as the representatives of nonpolar, semipolar and polar solvent. This study used vitamin C and vitamin E as positive controls. The percentage of antioxidant activity was obtained from the data absorbance. IC50 values obtained from linear regression between the concentration of tested series versus the percentage of antioxidant activity. The results showed that the ethanol extract of pidada fruit possessed very strong antioxidant activity while n-hexane fraction possessed weak antioxidant activity, ethyl acetate fraction possessed strong antioxidant activity, and butanol fraction possessed very weak antioxidant activity. IC50 values of ethanol extract, n-hexane fraction, ethyl acetate fraction, and butanol fraction of pidada fruit were 32,58; 191,31; 96,02 and 371,16 ppm respectively.